Composite

Part:BBa_K5061115

Designed by: Florent Cha   Group: iGEM24_Evry-Paris-Saclay   (2024-09-25)


ung gRNA transcription unit under the control of the J23119(SpeI) promoter

This part is a transcription unit of a guide RNA (gRNA) specific for the ung gene of E. coli under the control of the J23119(SpeI) promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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